Nishka Labs brings several years of experience of performing all types of analytical solutions. Our technical know-how and extensive experience with analysing and interpretation allow us to develop a range of authenticated results.
In vivo Activity
- DLA/EAC induced ascitic tumor model in mice
- DLA induced solid tumor model in mice
In Vitro Activity
- Cytotoxicity assay by MTT/SRB/Trypan blue
- Clonogenic assay
- Apoptosis – Nuclear staining with DAPI
- Alkylating activity assay
- TUNEL assay
- Endonuclease assay
- DNA fragmentation analysis / DNA ladder assay
- Cancer specific gene expression analysis
- P53, BCl2, Capsase 3, VEGF, cyclin D1, cyclin B1, STAT3 etc.
Anti Diabetes Activity
In vivo Activity
- Alloxan induced diabetic rat model
- Streptozotocin induced diabetic rat model
In Vitro Activity
- Glucose uptake studies in skeletal muscle cell lines (Non-radio labeled assay).
- Glucose uptake in isolated rat hemi diaphragm
- Gene expression studies (PPARγ, GLUT-4 etc)
- Alpha-Amylase inhibitory activity
- Alpha-galactosidase inhibitory activity
- Alpha-glucosidase activity
Hepatoprotective Activity
In vivo Activity
- Hepatoprotective study against selected toxicant in rats
In Vitro Activity
- Hepatoprotective study against selected toxicant.
- Hepatoprotective study in isolated primary rat hepatocytes.
- Gene expression studies
Anti infective Activity
In vivo Activity
- Different wound healing models in mice/rats
In Vitro Activity
- Anti microbial activity by Zone of inhibition.
- Anti microbial activity by MIC determination
- Antibiotic sensitivity testing
- TLC bioautography
- In vitro anti viral studies
Other in vivo services
- Hypercholesterolemia
- Arthritis
- Osteoporosis
- Benign prostatic Hyperplasia
- Spermatogenesis
- Allergy
- Antitussive
- Psoriasis
Other Services
- Antimicrobial
- Anti viral
- Cytotoxicity
- Cell proliferation
- Cancer
- Diabetes
- Hepatoprotection
- Obesity
- Immunomodulatory
- Inflammation
- Skin whitening
- Skin irritation (Dermal toxicity)
- Psoriasis
- Wound healing
- Genotoxicity
- Drug permeability
- Gene expression analysis
- Enzyme and Immunoassays (ELISA)
Antioxidant Activity
In vivo Activity
- Antioxidant status in rats by hepatic injury model
- Antioxidant status in tumor bearing mice model
In Vitro Activity
- DPPH scavenging assay
- Scavenging of Nitric Oxide radical
- Lipid peroxidation inhibitory activity
- Evaluation of Total Antioxidant capacity
- Ferric ion reducing power assay
- Scavenging of Hydroxyl Radical by p-NDA Method
- Scavenging of Hydrogen Peroxide
- Scavenging of ABTS radical cation Assay
- Total Phenolic and Flavonoid content estimation
Immunomodulatory & Inflammation Activity
In vivo Activity
- Carbon clearance assay model
- Cellular Immune Response Model
- Humoral Immune Response Model
- Neutrophil Adhesion Test Model
- Radiant heat method
- Tail immersion test
- Writhing test
- Paw edema model
- Oxazolone-induced ear edema in mice
In Vitro Activity
- Phagocytic assay on cellular lysosomal enzyme activity
- Proliferation assay
- NO and TNF inhibitory studies
- HRBC membrane stabilization
- Inhibition of protein denaturation
- Proteinase inhibitory action
- Analysis of markers like COX-1, COX-2, PGE-2, iNOS, IFN-alpha, IFN-gamma, TNF-alpha, Interleukin (IL)-1, IL-10, IL12, IL-18 etc.
Anti Obesity Activity
In vivo Activity
- Triton induced hyperlipidemia model in mice/rats
- Cocktail induced hypercholesterolemia model
In Vitro Activity
Anti adepogensis studies in 3T3L-1 pre adepocytes by
- Oil Red-O staining
- Cell triglyceride assay
- Gene expression studies (PPAR-gamma, SREBF etc)
Toxicology
- Cytotoxicity
- Genotoxicity
- AMES test
- Chromosomal aberration in CHO-K1 cells
- Micronucleus assay
- In vitro organ specific toxicity
- Skin irritation studies (as OECD)
- In vivo toxicity studies
Acute
Sub acute
Chronic - Specific organ toxicity
- Reproductive toxicity
